| Guaranteed Analysis | |
| Per Two 2.4 g Soft Chews | Min. |
| Moisture (Max) | 18.7% |
| *alpha-Amylase (Aspergillus oryzae) | 2,000 SKBU¹ |
| Lipase (Rhizopus oryzae) | 200 LU² |
| *Cellulase (Trichoderma longibrachiatum) | 70 CMCU³ |
| *Protease (Papaya) | 22,000 PU4 |
| *Total Microbes (Bacillus subtilis, Bacillus coagulans) | 1 Billion CFU |
| *Not recognized as an essential nutrient by the AAFCO Dog Food Nutrient Profiles. (All values are minimum unless otherwise noted.) (1) One SKB unit is defined as the amount of enzyme needed to form 10 mg of reducing sugars from 1% soluble starch solution at pH 5.0 and 40ºC in 30 minutes. (2) One FCC Lipase Unit (LU) is defined as the quantity of enzyme that will liberate ther equivalent of one μmol of acid (H+) per minute from the substrate, under the conditions of the assay. The assay is based on a five-minute hydrolysis of an olive oil substrate at pH 6.5 and 30ºC. The Fatty acids released on hydrolysis of the glycerol esters are determined by titration with sodium hydroxide. (3) One carboxymethyl cellulose unit (CMCU) is that amount of enzyme which liberates one micromole of reducing sugar (expressed as glucose equivalents) in one minute under the conditions of the assay. (4) The activity of papain is measured in the FCC PU- the assay of which is based on the hydrolysis of casein. The assay is based on a 60-minute proteolytic hydrolysis of a casein substrate at pH 6.0 and 40ºC. One PU (Papain Unit) is defined as that quantity of enzyme that liberates the equivalent of 1 μg of tyrosine per hour under the conditions of the assay. | |
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